Patent

  • 佐藤雄介、西澤精一、永富良一
    国際出願番号:PCT/JP2021/030716
    国際出願日:2021年8月23日
  • 西澤精一, 佐藤雄介, 芳野幸奈, 富樫奈央, 樋口啓
    名称:RNA検出用蛍光色素及びその使用方法
    出願番号: 特願2021-134803
    出願日:2021年8月20日
  • 西澤精一, 佐藤雄介, 芳野幸奈, 富樫奈央
    名称:RNA検出用蛍光色素及びその使用方法
    出願番号:特願2020-139795
    出願日:2020年8月21日
  • 佐藤雄介, 西澤精一, 田邊貴昭, 三浦弘真, オケケ シオマ ウシュ, 川口敦史
    名称:ウイルスRNAの複製と転写を阻害する複合体
    出願番号:特願2020-129884
    出願日:2020年7月31日
  • 佐藤雄介, 西澤精一, 田邉貴昭, 川口敦史
    名称:(仮)インフルエンザA型ウイルスRNA複製の制御方法
    出願番号:特願2019-141527
    出願日:2019年7月31日

  2021

  • 西澤精一, LEE En Ting Tabitha, 芳野幸奈, 矢島さやか, 六川正文, 佐藤雄介
    "rRNAを標的とする蛍光プローブの分子設計:FID法における蛍光インジケータと生細胞核小体イメージング蛍光色素"
    分析化学, accepted. (総合論文:「第81回分析化学討論会」特集号)

  • Yusuke Sato, Yugo Igarashi, Michiyuki Suzuki, Kei Higuchi, Seiichi Nishizawa
    "Deep-red fluorogenic cyanine dyes carrying amino group-terminated side chain for the improved RNA detection and nucleolar RNA imaging"
    RSC Adv., 56, 35051-35710 (2021). [DOI:10.1039/d1ra05872j]

  • Takaya Sato, Yusuke Sato, Seiichi Nishizawa
    "Spectroscopic, Thermodynamic and Kinetic Analysis of Selective Triplex Formation by Peptide Nucleic Acid with Double-stranded RNA over its DNA Counterpart"
    Biopolymers, accepted.

  • 伊東良子, 佐藤雄介, 寺前紀夫, 西澤精一
    "HIV-1 TAR RNAを標的とする蛍光インジケータ:モノメチンシアニン色素チアゾールオレンジの機能評価とFIDアッセイへの適用"
    分析化学, 70, 149-157 (2021). [DOI:10.2116/bunsekikagaku.70.149](化学センサー研究懇談会特集号)

  2020

  • En Ting Tabitha Lee, Yusuke Sato, Seiichi Nishizawa
    "Small Molecule-PNA Oligomer Conjugates for rRNA A-site at neutral pH for FID Assays"
    Chem. Commun., 56, 14976-14979 (2020). [DOI:10.1039/D0CC06084D] (Highlighted at Front Cover)

  • Yusuke Sato, Kazuki Kuwahara, Kenta Mogami, Kenta Takahashi, Seiichi Nishizawa
    "Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing"
    RSC Advances, 10, 38323-38327 (2020). [DOI:10.1039/d0ra07763a]

  • Chun-xia Wang, Yusuke Sato, Takashi Sugimoto, Norio Teramae, and Seiichi Nishizawa
    "Chloro-substituted naphthyridine derivative and its conjugate with thiazole orange for highly selective fluorescence sensing of an orphan cytosine in the AP site-containing duplexes"
    Applied Sciences, 10, 4133(2020). [DOI:10.3390/app10124133]
    (Invited to Special Issue "Nucleic Acids Conjugates for Biotechnological Applications")

  • Yusuke Sato, Yuki Takahashi, Takaaki Tanabe and Seiichi Nishizawa
    "Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA"
    Org. Biomol. Chem., 18, 4009-4013(2020). [DOI:10.1039/d0ob00794c]

  • Yusuke Sato
    "Design of fluorescent peptide nucleic acid probes carrying cyanine dyes for targeting double-stranded RNAs for analytical applications"
    Bull. Chem. Soc. Jpn., 93, 406-413(2020). [DOI:10.1246/bcsj.20190361]

  2019

  • Y. Yoshino, Y. Sato, S. Nishizawa
    "Deep-red light-up signaling of benzo[c,d]indole-quinoline monomethine cyanine for imaging of nucleolar RNA in living cells and for sequence-selective RNA analysis"
    Anal. Chem., 91, 14254-14260 (2019). [DOI:10.1021/acs.analchem.9b01997] (Highlighted at the Supplementary Cover Art)

  • Y. Sato, Y. Aiba, S. Yajima, T. Tanabe, K. Higuchi, S. Nishizawa
    "Strong binding and off-on signaling functions of deep-red fluorescent TO-PRO-3 for influenza A virus RNA promoter region"
    ChemBioChem., 20, 2752-2756(2019). [DOI:10.1002/cbic.201900331] (Highlighted at Cover Feature)

  • Y. Furuhata, M. Kobayashi, R. Maruyama, Y. Sato, K. Makino, T. Michiue, H. Yui, S. Nishizawa, K. Yoshimoto
    "Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation"
    RNA, 25, 590-599(2019).[DOI:10.1261/rna.069062.118]

  • Y. Sato, S. Yajima, A. Taguchi, K. Baba, M. Nakagomi, Y. Aiba, S. Nishizawa
    "Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA"
    Chemical Communications, 55, 3183 - 3186(2019). [DOI:10.1039/C9CC00414A] (Highlighted at Front Cover)

  2018

  • T. Tanabe, T. Sato, Y. Sato, S. Nishizawa
    "Design of a fluorogenic PNA probe capable of simultaneous recognition of 3’-overhang and double-stranded sequences of small interfering RNAs"
    RSC Advances, 8, 42095-42099(2018). [DOI:10.1039/C8RA08759H]

  • Y. Sato, M. Kaneko, T. Sato, S. Nakata, Y. Takahashi, S. Nishizawa
    "Enhanced binding affinity of siRNA overhang-binding fluorescent probes by conjugation with cationic oligopeptides for the improved analysis of siRNA delivery process"
    ChemBioChem., 20, 408-414(2019). [DOI:10.1002/cbic.201800560]

  • Y. Sato, M. Rokugawa, S. Ito, S. Yajima, H. Sugawara, N. Teramae, and S. Nishizawa
    "Fluorescent trimethylated naphthyridine derivative with an aminoalkyl side chain as the tightest non-aminoglycoside ligand for the bacterial A-site RNA"
    Chem. Eur. J., 24, 13862-13870(2018). [DOI: 10.1002/chem.201802320]
    [プレスリリース(東北大学大学院理学研究科HP)]

  • 佐藤雄介, 佐藤貴哉, 西澤精一
    "RNA結合性蛍光プローブの設計・合成と小分子RNA解析"
    分析化学, 67, 531-540(2018).(総合論文) [DOI: 10.2116/bunsekikagaku.67.531]

  • T. Masuda, Y. Shibuya, S. Arai, S. Kobayashi, S. Suzuki, J. Kijima, T. Itoh, Y. Sato, S. Nishizawa, A. Yamaguchi
    "Effect of Cavity Size of Mesoporous Silica on Short-DNA Duplex Stability"
    Langmuir, 34, 5545-5550(2018). [DOI: 10.1021/acs.langmuir.8b00437]

  • T. Sato, N. Sakamoto, S. Nishizawa
    "Kinetic and thermodynamic analysis of triplex formation between peptide nucleic acid and double-stranded RNA"
    Org. Biomol. Chem., 16, 1178-1187(2018). [DOI: 10.1039/C7OB02912H]

  • Y. Sato, D. Iwasawa, P. H. Kuo, R. Nakagomi, S. Nishizawa
    "Improved boronate affinity electrophoresis by optimization of the running buffer for a single step separation of piRNA from mouse testis total RNA"
    Anal. Sci., 34, 627-630(2018). [DOI : 10.2116/analsci.17N024]

  2017

  • T. Chiba, T. Sato, Y. Sato, S. Nishizawa
    "Red-emissive triplex-forming PNA probes carrying cyanine base surrogates for fluorescence sensing of double-stranded RNA"
    Org . Biomol. Chem., 15, 7765-7769 (2017). [DOI: 10.1039/C7OB02077E]

  • T. Sato, Y. Sato, S. Nishizawa
    "Optimization of the Alkyl Linker of TO Base Surrogate in Triplex-Forming PNA for Enhanced Binding to Double-stranded RNA"
    Chem. Eur. J., 23, 4079–4088 (2017). [DOI:10.1002/chem.201604676] (Highlighted on the ChemistryViews website, Selected as Hot Paper, Highlighted at Inside Back Cover)

  2016

  • Y. Sato, H. Saito, D. Aoki, N. Teramae, S. Nishizawa
    "Lysine linkage in abasic site-binding ligand–thiazole orange conjugates for improved binding affinity to orphan nucleobases in DNA/RNA hybrids "
    Chem. Commun., 52, 14446-14449 (2016). [DOI: 10.1039/C6CC07236D]

  • T. Sato, Y. Sato, S. Nishizawa
    "Triplex-forming Peptide Nucleic Acid Having Thiazole Orange as a Base Surrogate for Fluorescence Sensing of Double-stranded RNA "
      J. Am. Chem. Soc., 138, 9397-9400 (2016). [DOI: 10.1021/jacs.6b05554]

  • T. Suzuki, Y. Shibuya, T. Sato, S. Nishizawa, I. Sato, A. Yamaguchi
    "Thermodynamics of Complexation between Thiourea-based Receptor and Acetate in Water/Acetonitrile Mixture"
      Anal. Sci., 32, 741-744 (2016). [DOI: 10.2116/analsci.32.741 ]

  • Y. Sato, T. Asami, Y. Toriyabe, T. Sato, N. Teramae, S. Nishizawa
    "Pteridine Derivatives Modified with a Guanidine for Binding and Sensing an Orphan Guanine in RNA duplexes "
      Chem. Lett., 45, 982-984 (2016). [DOI: 10.1246/cl.160425]

  2015

  • T. Sato, Y. Sato, K. Iwai, S. Kuge, N. Teramae, S. Nishizawa
    "Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe"
      Anal. Sci., 31, 315-320 (2015). [DOI: 10.2116/analsci.31.315]
  • T. Sato, Y. Sato, K. Iwai, S. Kuge, S. Nishizawa, N. Teramae
    "Synthetic fluorescent probes capable of selective recognition of 3'-overhanging nucleotides for siRNA delivery imaging"
      Chem. Commun., 51, 1421-1424 (2015). [DOI: 10.1039/C4CC08800J](Back Cover)

  • J. Tao, P. Song, Y. Sato, S. Nishizawa, N. Teramae, A. Tong, Y. Xiang
    "A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity"
      Chem. Commun., 51, 929-932 (2015). [DOI: 10.1039/C4CC06170E]

  2014

  • Y. Sato, T. Sato, T. Sato, S. Nishizawa, N. Teramae
    "Effect of LNA nucleobases as an enhancer for the binding of amiloride to an abasic site in DNA/DNA and DNA/RNA duplexes"
      Org. Biomol. Chem., 12, 7250-7256 (2014). [DOI: 10.1039/C4OB00977K]

  • Y. Sato, M. Kudo, Y. Toriyabe, S. Kuchitsu, C.-X. Wang, S. Nishizawa, N. Teramae
    "Abasic Site-Binding Ligands Conjugated with Cyanine Dyes for "Off-On" Fluorescence Sensing of Orphan Nucleobases in DNA Duplexes and DNA/RNA Hybrids"
      Chem. Commun., 50, 515-517 (2014). [DOI: 10.1039/C3CC47717G](Front Cover)


  • S. Nishizawa, Y. Sato, N. Teramae
    "Recent Progress in Abasic Site-binding Small Molecules for Detecting Single-base Mutations in DNA"
      Anal. Sci., 30, 137-142 (2014). [DOI: 10.2116/analsci.30.137](Invited review)

  2013

  • Y. Sato, Y. Toriyabe, S. Nishizawa, N. Teramae
    "2,4-Diamino-6,7-dimethylpteridine as a Fluorescent Ligand for Binding and Sensing an Orphan Cytosine in RNA Duplexes"
      Chem. Commun., 49, 9983-9985 (2013). [DOI: 10.1039/C3CC46085A]

  • A. Futamura, A. Uemura, T. Imoto, Y. Kitamura, H. Matsuura, C.-X. Wang, T. Ichihashi, Y. Sato, N. Teramae, S. Nishizawa, T. Ihara
    "Rational Design for Cooperative Recognition of Specific Nucleobases Using β-Cyclodextrin-Modified DNAs and Fluorescent Ligands on DNA and RNA Scaffolds"
      Chem. Eur. J., 19, 10526-10535 (2013). [DOI: 10.1002/chem.201300985]

  • Y. Sato, T. Kageyama, S. Nishizawa, N. Teramae
    "Competitive binding of abasic site-binding ligands and masking ligands to DNA duplexes for the analysis of single base mutation"
      Anal. Sci., 29, 15-19 (2013). [DOI: 10.2116/analsci.29.15]

  Review Article


  Book

  • 本水昌二・朝本紘充・石坂昌司・井原敏博・内山一美・齊藤和憲・佐藤健二 塚原 聡・中釜達朗・西澤精一・沼田 靖・南澤宏明・森田孝節・吉川賢治
    基礎教育シリーズ 分析化学 機器分析編(第2版)
    東京教学社(2021)[ISBN 978-4-8082-3059-3]

  • 本水昌二・朝本紘充・石坂昌司・井原敏博・内山一美・齊藤和憲・佐藤健二 塚原 聡・中釜達朗・西澤精一・沼田 靖・南澤宏明・森田孝節・吉川賢治
    基礎教育シリーズ 分析化学 基礎編(第2版)
    東京教学社(2021)[ISBN 978-4-8082-3058-6]

  • [監修] 日本核酸化学会 [編] 杉本直己
    核酸科学ハンドブック
    講談社(2020)[ISBN: 978-4-06-520786-4]
    (第II部 核酸科学の最前線 7. 非ワトソンークリック塩基対結合分子 西澤精一)

  • G. D. Christian, P. K. Dasgupta, K. A. Schug 著 今任稔彦・角田欣一監訳(分担翻訳)
    クリスチャン分析化学 原書7版 II. 機器分析編
    丸善出版(2017)[ISBN: 978-4-621-30110-4]

  • G. D. Christian, P. K. Dasgupta, K. A. Schug 著 今任稔彦・角田欣一監訳(分担翻訳)
    クリスチャン分析化学 原書7版 I. 基礎編
    丸善出版(2016)[ISBN: 978-4-621-30109-8]

  Others

  • 西澤精一、佐藤雄介
    「RNA解析・生細胞イメージングのための深赤色蛍光色素」
    ぶんせき、2020、450-454.
    (第11号、特集 生命現象の”見える化"を支える分析化学)
  • リー エン ティン タビサ
    「グラフェン酸化物を利用した抗HIV薬剤の探索」
    ぶんせき, 2020 第4号 トピックス, 132.
  • 佐藤貴哉, 佐藤雄介, 西澤精一
    「RNA解析・イメージングのための蛍光性核酸プローブの分子設計」
    ぶんせき, 2017, 459-463.
  • 佐藤貴哉, 佐藤雄介, 西澤精一
    「RNA二重鎖を読み解く新技術-三重鎖核酸形成を利用する蛍光プローブ」
    月刊「化学」72巻4月号, 47-52




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